Knockdown of ANXA11 expression in GC cell lines. (A) Effect of silencing of ANXA11 using different siRNA. GC cell lines were treated with ANXA11-siRNA1, ANXA11-siRNA2, ANXA11-siRNA3, and NC-siRNA at the concentration of 40 nM or without treatment (Con) for 24 h or 48 h. (B) Dose-dependent silencing of ANXA11 by SGC-7901 cells were treated with ANXA11-siRNA1 at the indicated concentrations of 100 nM, 80 nM, 60 nM, 40 nM, 20 nM, or NC at the concentration of 40 nM or without treatment. (C, D) WB and qRT-PCR were performed to check the ANXA11 expression levels in AGS and SGC-7901 cell lines with ANXA11-siRNA at the concentration of 40 nM. * P<0.05.