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. 2017 Oct 25;15(1):83–90. doi: 10.3892/ol.2017.7266

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Copyright: © Chen et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Wnt4 mRNA expression following candidate plasmid transfection. (A) Agarose gel electropherogram of Wnt4 mRNA expression following transfection. (B) Evaluation of mRNA expression levels showed that plasmid shRNA-Wnt4 effectively silences Wnt4 expression in the latter three groups, but shRNA-Wnt4-3 was most effective. Therefore, shRNA-Wnt4-3 was selected as the best interference plasmid and used in subsequent experiments. *P<0.05 and **P<0.01 vs. control group. shRNA, short hairpin RNA; Lane M, marker; lane 1, control; lane 2, TR001; lane 3, shRNA-Wnt4-1; lane 4, shRNA-Wnt4-2; lane 5, shRNA-Wnt4-3; and lane 6, shRNA-Wnt4-4.