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Wnt 4 and JNK mRNA and protein expression subsequent to shRNA-Wnt4-3 transfection. These results indicated that the shRNA-Wnt4 plasmid inhibits JNK mRNA expression, which suppresses Wnt4 and JNK protein expression. (A) Agarose gel electropherogram of JNK mRNA after Wnt4 shRNA. (B) Relative expression of Wnt4 and JNK mRNA subsequent to plasmid transfection. *P<0.01 vs. control group. (C) Wnt4 and JNK protein expression. JNK, c-Jun N-terminal kinase.
