Figure 4.

Loss of TRB3 increased the phosphorylation of AKT in HCC cells. (A) AKT and pAKT expression levels in cells transfected with the most efficient lentivirus containing TRB3- and NC-shRNA for 24 h were analyzed by western blot anlysis. (B) Analysis of TRB3 mRNA expression levels in MHCC97H cells transfected with lentivirus containing TRB3- and NC-shRNA by reverse transcription-quatitative polymerase chain reaction. *P<0.05, **P<0.01. AKT, protein kinase B; TRB3, tribbles homolog 3; p, phosphorylated; HCC, hepatocellular carcinoma; NC, negative control; sh, short hairpin; B, blank control.