Table I.
Gene | Primer sequence | Product size, bp |
---|---|---|
β-actin (13) | 162 | |
Forward | 5′-GACTTAGTTGCGTTACACCCTTTCT-3′ | |
Reverse | 5′-GAACGGTGAAGGTGACAGCAGT-3′ | |
HIF-1α (14) | 150 | |
Forward | 5′-TCTGGGTTGAAACTCAAGCAACTG-3′ | |
Reverse | 5′-CAACCGGTTTAAGGACACATTCTG-3′ | |
CXCR4 (14) | 184 | |
Forward | 5′-TCTGTGACCGCTTCTACC-3′ | |
Reverse | 5′- AGGATGAGGATGACTGTGG-3′ | |
VEGF (14) | 176 | |
Forward | 5′-TGCTTCTGAGTTGCCCAGGA-3′ | |
Reverse | 5′-TGGTTTCAATGGTGTGAGGACATAG-3′ |
HIF-1α, hypoxia-inducible factor-1α; CXCR4, C-X-C motif chemokine receptor 4; VEGF, vascular endothelial growth factor.