Figure 7.

Subcellular localization and transcriptional activity of OsWRKY67. (A) Nuclear localization of the OsWRKY67-GFP in maize mesophyll protoplasts. Photographs were taken in the dark field for green fluorescence and in the bright field for cell morphology. OsABF1-RFP is a nuclear marker. Chlorophyll autofluorescence was used as a chloroplast marker. OsWRKY67-GFP signals are green, and nuclear signals are red. (B) Schematic representation of constructs used for analysis of GAL4-responsive transcription by OsWRKY67 fused to the GAL4 BD in maize protoplasts. ZmUBQ1:GUS was used as an internal control. (C) Relative luciferase activities in maize protoplasts after cotransfection with the reporter, effector, and control plasmids. The fold changes were calculated as the LUC/GUS activity ratio. LUC activities were normalized after transfection with the empty vector (EV) control (arbitrarily set at 1). Asterisks represent statistical significance with Student's t-test, p < 0.0001.