Figure 3.

ICG-001 inhibits the expression of genes involved in the mTOR pathway, Wnt pathway, and stemness. (A, B) GSEA shows negative enrichment of transcriptional targets of mTORC1 and Wnt signaling pathways (MSigDB curated geneset). (C, D) GSEA of a WNT5A siRNA signature, derived from a prostate cancer cell line (LNCaP-abl), compared against the ICG-001 signature from Mel202 cells shows strong concordance. (E) Normalized enrichment scores from stemness gene sets (from MSigDB) compared against the ICG-001 transcriptional footprint. Strong inhibition of embryonic stem cell (ESC)-associated transcriptional programs is observed upon ICG-001 treatment of UM cells. Gene sets with a nominal P value <0.05 and FDR < 0.25 were defined as significantly enriched.