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. 2018 Jan 16;16:4. doi: 10.1186/s12951-017-0330-1

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 8 — a Fluorescence images on the platform of the IsoFlux System, resulting from cytokeratin (green fluorescence), leukocyte (red fluorescence), and nucleus marker staining, and phase contrast image superimposed on the fluorescence image for an example of CTC recognition. b Fluorescence images on the EBFM assay, resulted from the FITC-conjugated anti-EpCAM (green fluorescence), Alexa594-conjugated anti-Ck8+18 (red fluorescence), and leukocyte marker (CD45) staining, and phase contrast image superimposed on the fluorescence image for an example of CTC recognition. c CTC counts for seven subjects, obtained using IsoFlux system, N50/P50 fibrous mats with anti-EpCAM grafts