Fig. 2.

Different L. major stages incubated with sand fly midguts dissected at 24 h post-blood meal. Leishmania major amastigotes (0 h AMA), parasites within amastigote-promastigote transition (5 h AMA/PROMA) and promastigotes (24 h PROMA) were incubated in a microtiter plate for 2 h with saline or midguts of P. argentipes, P. orientalis, P. papatasi and S. schwetzi dissected at 24 h post-blood meal. As a positive control, parasites killed by 1% formaldehyde and permeabilised by 0.5% Triton X-100 were used