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. 2018 Jan 15;15:12. doi: 10.1186/s12985-018-0928-4

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — pUL41 intracellular localization in DEV-infected cells at different times. a Representative confocal immunofluorescence microscopic images of the UL41 localization (green in all images) at 7, 12, 24, 36, 48, 60, and 72 hpi. Nuclei are indicated in blue (DAPI). The control group comprised untreated DEF cells (magnification: 200×; scale bar: 50 μm) detected with mouse anti-UL41 antibody serum (1:800) and goat anti-mouse IgG (H + L) cross-adsorbed secondary antibody, Alexa Fluor 488 (1:1000). Significantly higher UL41 levels in the cytoplasm were evident in the DEF cells infected with wild-type DEV