Table 1.
Sequences and primer pair characteristics
| Primer | Primer sequence (5′ → 3′) | Gene | Product size (bp) |
|---|---|---|---|
| P1 | G/AATTCATGGGGCTGTATGGTTGTATAAGC | DEV UL41 | 1526 |
| P2 | C/TCGAGTCTTACAACAGTTAATCTTAGTCCCAAT | ||
| P3 | G/AATTCGCCACCATGGGGCTGTATGGTTGTATAAGC | DEV UL41 | 1532 |
| P4 | G/GTACCTCTTACAACAGTTAATCTTAGTCCCAAT | ||
| P5 | CGCG/GATCCGCCACCATGGATAAATCACGAAGACAGCG | DEV UL47 | 2391 |
| P6 | CCGC/TCGAGTTAATGTAACTCTCTCCGCCCAG | ||
| P7 | TGATTTACACCGCTACCCTA | DEV UL41(RT-PCR) | 107 |
| P8 | TCTCACTTCTTTCAGCCATT | ||
| P9 | CCGGGCATCGCTGACA | Duck β-actin(RT-PCR) | 177 |
| P10 | GGATTCATCATACTCCTGCTTGCT | ||
| P11 | GAACAACCGCCGAACAC | DEV UL54 | 127 |
| P12 | TCAAACATCCGCCTCAA | ||
| P13 | GCCACCAACCCTACCAAG | DEV UL13 | 131 |
| P14 | GTCGTCAGCCCATCACCA | ||
| P15 | AGACGGTTCCGAAAGTACAG | DEV Us2 | 111 |
| P16 | TCGGCAGCACCAATAATCC |