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Schematic diagram of the in vitro tear turnover apparatus. The model incorporates an insert containing iHCECs as turnover chamber; the external basal side of the insert is sealed to avoid down diffusion of material. The temperature of the system is 34°C (the human tear film temperature). Two peristaltic pumps were used to control the inflow and outflow of simulated tears in the chamber.
Abbreviation: iHCECs, human immortalized cornea epithelial cells.
