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. 2017 Dec 15;130(24):4108–4119. doi: 10.1242/jcs.207134

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© 2017. Published by The Company of Biologists Ltd

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Fig. 3. — CIF1-CTD forms a 1:1 stoichiometric complex with CIF2-NTD. Size-exclusion chromatography (S200 16/60) elution profiles of three different combinations of co-purified CIF1-CTD (aa 667–804, 15.2 kDa) and CIF2-NTD (aa 1–160, 17.4 kDa) are overlaid to show the shifts of the larger complexes with one or both proteins bearing a SUMO tag. Peak fractions from each elution were examined on an SDS-PAGE gel (bottom), which showed that the two proteins are co-eluted and have a 1:1 molar ratio in each case.