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. 2017 Dec 1;10(12):1433–1438. doi: 10.1242/dmm.029868

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© 2017. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 1. — Protocol diagram summarizing the number of animals included, with exclusions per group, for final analysis. Two experimental groups were compared: (A) animals evaluated with only laser Doppler monitoring; (B) animals evaluated with laser Doppler monitoring, MR angiography and DWI. The two groups were compared in animals with and without occlusion of the pterygopalatine artery. (C) Infarct volume determined 24 h after ischaemia for the two inclusion protocols used in rats with and without pterygopalatine artery occlusion. Data are expressed as mean±s.d. Student's t-test was used to compare the differences between the means, and F-test was used to compare differences in variability. Means were similar for both inclusion protocols, while the variability was significantly reduced with the new inclusion protocol suggested (*P<0.05).