Fig. 6.

Fgfr3;4 mutant lungs display an increase in Mfap5, Igf1 and Fbn2 expression. (A) qRT-PCR of genes involved in elastogenesis. The expression of Mfap5, Igf1 and Fbn2 was increased in the Fgfr3;4 mutant lung at P2 (*P<0.05, n=3 each). Mean±s.e.m. (B-D) Representative immunofluorescent staining for MFAP5 (green), the myofibroblast marker αSMA (red) and nuclei (DAPI, blue) at P7. Scale bars: 100 μm. (E) Western analysis indicates that MFAP5 (green) is also increased at the protein level in Fgfr3;4 mutants as compared with the control at both P2 and P8. β-actin (red) was used as a loading control.