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. 2017 Dec 15;144(24):4510–4521. doi: 10.1242/dev.152504

Fig. 3.

Fig. 3.

Effect of lncRNA depletion on MSC chondrogenic differentiation. (A-D) MSCs were transfected for 2 days with SOX9-AS1 or ROCR-targeting or non-targeting control (siCon) siRNA prior to chondrogenic differentiation in hanging transwell inserts. (A) SOX9-AS1 and ROCR expression in RNA extracted from Day 3 and Day 7 chondrogenic discs. Expression is presented as a percentage of non-targeting control levels. (B) Representative Day 3 and 7 chondrogenic discs. (C) Wet mass of Day 7 chondrogenic discs. (D) Representative Safranin O staining of Day 7 chondrogenic discs. (E-G) MSCs were transfected for 1 day with ROCR-targeting or non-targeting control siRNA prior to chondrogenic differentiation in a V-bottom 96-well plate. (E) Representative Day 7 chondrogenic pellets. (F) GAG levels assayed by DMB assay in Day 7 chondrogenic pellets. (G) DNA quantification by PicoGreen assay in Day 7 chondrogenic pellets. Values are mean±s.e.m. of data pooled from three (A-D) or four (E-G) MSC donors. *P<0.05; ***P<0.001 for lncRNA siRNA versus non-targeting siRNA. Significant differences between sample groups were assessed by one-way analysis of variance followed by the Bonferroni post-hoc test for multiple comparisons or a two-tailed Student's t-test was performed for single comparisons.