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. 2017 Dec 15;144(24):4510–4521. doi: 10.1242/dev.152504

Fig. 5.

Fig. 5.

Effect of lncRNA depletion on MSC osteoblastogenic and adipogenic differentiation. (A-C) MSCs were transfected for 2 days with SOX9-AS1 or ROCR-targeting or non-targeting control (siCon) siRNA prior to osteoblastogenic differentiation. (A) Representative matrix mineralisation assayed by Alizarin Red staining after 21 days. (B) Quantification of Alizarin Red staining. (C) RNA was extracted and expression of the indicated genes at Day 7 measured by real-time RT-PCR. (D-F) MSCs were transfected for 2 days with SOX9-AS1 or ROCR-targeting or non-targeting control siRNA prior to adipogenic differentiation. (D) Representative fat droplet generation assayed by Oil Red O staining after 14 days. (E) Quantification of Oil Red O staining. (F) RNA was extracted and expression of the indicated genes at Day 7 measured by real-time RT-PCR. Values are mean±s.e.m. of data pooled from four MSC donors. *P<0.05; ***P<0.001 for lncRNA siRNA versus non-targeting siRNA. Significant differences between sample groups were assessed by one-way analysis of variance followed by the Bonferroni post-hoc test for multiple comparisons or a two-tailed Student's t-test was performed for single comparisons.