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. 2017 Dec 15;144(24):4540–4551. doi: 10.1242/dev.156349

Fig. 1.

Fig. 1.

Identification of two classes of molecular markers for planarian pigment cells. (A) Whole-worm RNA samples were collected at five time points: before light exposure (‘WT’); exposed to light treatment for 8 days (D8); recovered in darkness for 1 day (R1), 2 days (R2) or 8 days (R8). (Top) Bright-field images of animal pigmentation status at time points WT, D8 and R8. Animals were fully depigmented at D8, whereas animals were partially repigmented at R8. (Bottom) Expression profile of 50 genes with the greatest downregulation at D8, in descending order of fold decrease. (B,C) WISH of candidate pigment cell markers. Six genes show dendritic expression patterns (B) and four genes show punctate expression patterns (C) by WISH. Top rows show that dendritic genes have varying degrees of expression in the subepidermal layer, whereas punctate genes have an even distribution across the animal in the subepidermal layer. Bottom rows show that gene expression is undetectable by WISH in depigmented animals at D8. (B′) Higher magnification image of neck region (boxed region in B), showing individual cells with dendritic expression of PBGD-1. (C′) Higher magnification image of neck region (boxed region in C), showing individual cells with punctate expression of lysoLP-1. Scale bars: 250 μm in A,B,C; 100 μm in B′,C′.