Fig. 5.

Embryonic type II neuroblasts express late temporal transcription factors. (A-F) Temporal transcription factor expression in the earliest type II neuroblast to form (posterior-most, see Fig. 1). Type II neuroblasts are identified as Dpn⁺ Ase⁻ (left columns); temporal transcription factor expression reveals sequential expression of Pdm⁺→Pdm⁺/Cas⁺→Cas⁺→Cas⁺/Grh⁺→Grh⁺. Schematics on the right summarize this cascade. Later-forming type II neuroblasts start the cascade with Cas. Yellow circles indicate the small Grh⁺ neuroblast. Scale bar: 10 μm. n>10 for the experiment shown.