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. 2017 Nov 3;6(12):1861–1868. doi: 10.1242/bio.026963

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© 2017. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — A dominant-negative fzd7 induces cardia bifida phenotype. (A,Ai,F,Fi) Lateral and ventral views of wild-type embryos at stage 29 showing normal tnnic (A, Ai) and nkx2-5 (F-Fi) expression in the heart. (B,Bi,G,Gi) Lateral and ventral views of embryos injected in the DB at 4-cell stage with dominant-negative fzd7 (fzd7 CRD). The cardia bifida phenotype is shown by tnnic (B,Bi) and nkx2-5 (G,Gi) expression. These embryos were fixed at the same stage as the control embryos in A and F. (C) Graph showing fzd7 CRD cardia bifida phenotype percentages indicated by tnnic expression. (D,Di) Lateral and ventral views of embryos injected in the DB at 4-cell stage with full-length of fzd7 (fzd7 FL) showing normal heart tube. Note that embryos in D and G are showing severe convergent extension defects but cardia bifida phenotype is only induced by fzd7 CRD. (E,Ei) Lateral and ventral views of injected embryo in the DB at 4-cell stage with fzd3 dominant-negative form (fzd3 CRD) showing normal heart looping at stage 38 indicating that fzd7 CRD cardia bifida phenotype is specific to fzd7. Magnification 20×.