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. 2017 Nov 14;6(12):1904–1912. doi: 10.1242/bio.028548

Fig. 4.

Fig. 4.

SiRNA-mediated inhibition of STMN1, JIP1 and JNK3 expression and its effects on cell proliferation, apoptosis and cell cycle progression in NMR muscle fibroblasts. (A) Real-time PCR analysis of STMN1, JIP1 and JNK3 mRNA levels in fibroblasts transfected with siRNA for 24 h under hypoxic conditions. GAPDH was used as an internal reference. (B) Detection of STMN1, JIP1 and JNK3 protein in fibroblasts after transfection with siRNA for 48 h under hypoxic conditions. (C) Effects of inhibition of STMN1, JIP1 and JNK3 for 24 h, 48 h, or 72 h on the growth of NMR fibroblasts. (D) Effect of inhibition of STMN1, JIP1 and JNK3 for 48 h on cell apoptosis. (E) Statistical analysis of NMR fibroblast apoptosis after transfection with siRNA for 48 h. (F) Flow cytometric (FCM) analysis of the cell cycle distribution of NMR fibroblasts after transfection with siRNA for 48 h. (G) Quantification of FCM results in the different groups. ★P<0.05, ★★P<0.01 (t-test). Data in A, C E and G are represented as mean±s.e.m.