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. 2018 Feb;24(2):183–195. doi: 10.1261/rna.063479.117

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© 2018 Zhao et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article, published in RNA, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.

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FIGURE 3. — Comparison of RT products from MarathonRT, SSIV, and TGIRT using the HCV genome as template. (A) A representative denaturing alkaline agarose gel showing products from multiple-turnover RT reactions catalyzed by different polymerases. (SSIV) Superscript IV. The ratio of signal intensity from full-length product divided by total products for each polymerase are indicated under each gel lane, and were calculated as described in Materials and Methods. (B) Intensity profile for gel lanes in A that represent RT products produced by MarathonRT, SSIV, and TGIRT. RT reactions for SSIV and TGIRT were performed with optimal temperature (55°C for SSIV and 60°C for TGIRT) and standard buffer conditions according to the manufacturer's protocol.