FIGURE 5.

Expression of asXUTs is associated to changes of histone modifications patterns. (A) Metagene view of H3K14ac levels in WT cells for genes with (red lines) or without (blue lines) asXUTs. Biological duplicates of YAM2400 (WT) cells were grown to mid-log phase in rich medium. After cross-linking, chromatin extraction and sonication, histone H3 and H3K14ac were immunoprecipitated. Coprecipitated DNA fragments were purified and used to construct ChIP-seq libraries. Metagene representation of signal for each class of genes was performed as above, in a strand-unspecific manner, using ratio of coverage (log₂) for H3K14ac and H3. The shading surrounding each line denotes the 95% confidence interval. (B) Same as above for H4K5/8/12/16 acetylation (H4ac). (C) Metagene view of H3K4me3 levels in WT cells for genes with (red lines) or without (blue lines) asXUTs. Analysis was performed as described above, using previously published ChIP-seq data for H3K4me3 and H3 (DeGennaro et al. 2013). Data are presented as above. (D) Same as above for H3K36me3, using previously published ChIP-seq data for H3K36me3 and H3 (DeGennaro et al. 2013).