Figure 3.

Wdr82 is required for Set1A recruitment to chromatin and H3K4me3. (A) Pie chart showing the percentage of Set1A peaks found in different regions of the genome. (B) GO analysis of genes whose promoters are bound by Set1A. Groups corresponding to one of the most highly expressed gene clusters in HPCs (Supplemental Fig. S1B) are highlighted in red. (C) ChIP-seq traces showing Set1A or H3K4me3 binding to chromatin in control knockdown or Wdr82 knockdown cells. Red arrows indicate regions of interest. (D) ChIP-seq traces showing H3K4me3 binding to genomic regions in control knockdown or Wdr82 knockdown cells. (E) Genome-wide histogram showing the binding of H3K4me3 in relation to promoter regions for the experimental conditions indicated.