Figure 4.

Nup98 is required for Set1A recruitment to chromatin and H3K4me3. (A) Model showing the potential role of Nup98–Wdr82 interaction in HPCs. Perhaps Nup98 is required to recruit the WSC complex to chromatin. (B) Overlap of Set1A promoter peaks with Nup98 promoter peaks from experiments conducted in mouse HPCs. (C) Genome-wide histogram showing the binding of Nup98, Set1A, or a control ChIP (IgG) in relation to promoter regions in wild-type HPCs. (D) ChIP-seq trace showing Nup98 binding, Set1A binding in control or Nup98 knockdown conditions, or H3K4me3 binding in control, Nup98 knockdown cells, or Wdr82 knockdown cells in relation to the Hjurp gene promoter. Note that Nup98 knockdown was carried out for 24 h, while Wdr82 knockdown was carried out for 48 h except where indicated in D. (E) Genome-wide histogram showing the binding of Set1A in relation to promoter regions in control knockdown (blue) or Nup98 knockdown (red) cells. (F) Graph showing the number of promoters bound by Set1A in control knockdown or Nup98 knockdown cells. (G) Genome-wide histogram showing the binding of H3K4me3 in relation to promoter regions in control knockdown (blue), Nup98 knockdown (red), or Wdr82 knockdown (yellow) or for a control IgG ChIP (brown). Dotted traces indicate data that are the same as shown in Figure 3E.