Figure 3. JAK 1/2 inhibition following bone marrow transplantation mitigates CD11b⁺/GR1⁺-mediated low-grade glioma transformation.

(A) Schematic of RCAS low grade murine glioma bearing animals post irradiation (2wks) and GFP⁺ bone marrow transplantation, treated with AZD1480. (B) Representative image of T2-weighted 7T MRI of vehicle control (n=7) and AZD1480 treated (n=7) animals at 10 weeks. Animals were imaged around 7 weeks. (C) Representative photomicrographs of tumor cross sections from mice treated with vehicle control (n=5) and AZD1480 (n=5). Quantification of Average number of GFP cells per high power field in bar graph format, (n=10),Scale bar 50μm, **p=0.004. (D)Representative photomicrographs of tumor cross sections from mice treated with vehicle control (n=5) and AZD1480 (n=5) co-stained with GFP⁺ and CD11b⁺. Quantification of average number of cells per high power field in bar graph format labeled with GFP⁺, CD11b⁺ and double labeled with GFP⁺/CD11b⁺, (n=10), Scale Bar 50μm, *p<0.05.