Figure 4. TGFβ signaling induces fibronectin upregulation and macrophage recruitment to the liver pre-metastatic niche.
Immunofluorescence quantification of FN and αSMA expression in arbitrary units (A.U.) and F4/80+ cells in livers of mice educated with PBS (CTL), PAN02 exosomes alone (Exo), or in combination with the TGFβ receptor inhibitor A83-01 (Exo+A83-01); n = 4 (CTL) and n = 7 (Exo and Exo+A83-01) mice pooled from two experiments. The data are represented as mean±s.e.m. ***P < 0.001, N.S. stands for not significant by ANOVA. Scale bars, 200μm.