Figure 2.

Trehalose, glycerol, ectoine 1°C/min DE algorithm results for Jurkat cells. (A) Cumulative best member solution; recovery associated with the best solution increases and plateaus as the algorithm converges. (B) Number of improved solutions/generation; the number of improved solutions in each generation decreases and reaches zero when the algorithm has converged. (C) Emergent population with the generational average overlaid: the emergent population improves and eventually stops changing as the DE algorithm converges; this is reflected in the generational average, which increases and begins to plateau as the algorithm converges. The optimum composition identified by this run of the algorithm was 150 mM trehalose, 10% glycerol, 0.1% ectoine for Jurkat cells frozen at 1°C/min