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. Author manuscript; available in PMC: 2018 Jan 16.
Published in final edited form as: J Tissue Eng Regen Med. 2016 May 27;11(10):2806–2815. doi: 10.1002/term.2175

Figure 3.

Figure 3

Trehalose, glycerol, ectoine cooling-rate DE algorithm results for Jurkat cells. (A) Cumulative best member solution; this increases and plateaus as the DE algorithm converges. (B) Number of improved solutions/generation; this decreases until it reaches zero as the DE algorithm converges. (C) Emergent population with the generational average overlaid: pie charts at each average show the cooling rate distribution within each generation. The optimum composition identified by this run of the DE algorithm was 300 mM trehalose, 10% glycerol, 0.01% ectoine at 10°C/min cooling