Sucrose, ethylene glycol, alanine, taurine, ectoine cooling-rate algorithm results for MSCs. (A) Cumulative best member solution; this increases and plateaus as the DE algorithm converges. (B) Number of improved solutions/generation; this decreases until it reaches zero as the DE algorithm converges. The optimum composition identified by this run of the DE algorithm included 300 mM ethylene glycol, 1 mM taurine, 1% ectoine with a 1°C/min cooling rate