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. Author manuscript; available in PMC: 2018 Jan 16.
Published in final edited form as: J Tissue Eng Regen Med. 2016 May 27;11(10):2806–2815. doi: 10.1002/term.2175

Figure 6.

Figure 6

Scale-up viability and recovery of Jurkat cells (A, B) and mesenchymal stem cells (C, D) frozen in DE algorithm-optimized solutions. Each cell type was frozen at 10°C/min in a TGE solution optimized for Jurkat cells (TGE 10°C) and a SEGA solution optimized for MSCs (MSC 10°C). Results were compared to cells frozen in DMSO at 1°C/min (DMSO 1°C), as it represents the current gold standard for both cell types. Jurkat cells performed well in both the SEGA 10°C and TGE 10°C solutions, and Jurkat cell viability was significantly higher in TGE 10°C than both SEGA 10°C and the DMSO 1°C control (A). MSCs also performed well in both SEGA 10°C and TGE 10°C solutions, and had significantly higher recovery in the SEGA 10°C solution than both TGE 10°C and the DMSO 1°C control (D). Significance markers indicate p < 0.05