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. 2018 Jan 16;13(1):e0191372. doi: 10.1371/journal.pone.0191372

Fig 2. H69D mutation abolishes autoprocessing in the context of L-MBP/F-MBP fusion independent of p6* sequences.

Fig 2

(A) Schematic illustration of the tested constructs. (B) Autoprocessing of H69 mutants in the context of MBP fusions. Approximately equal amounts of lysate were probed with mouse anti-Flag and anti-HA antibodies in parallel. The corresponding proximal (P) and distal (D) processing products are denoted and connected by the solid and dashed lines, respectively. (C) Autoprocessing of L-MBP fusion precursors carrying p6* truncations.