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. 2018 Jan 16;13(1):e0191277. doi: 10.1371/journal.pone.0191277

Fig 1. Composition, amplification and gene cloning.

Fig 1

Tandem repeat gene of RSV G glycoprotein was designed as indicated (A). For V1 VLPs, 1’ tandem includes between 1 and 260 amino acids of G glycoprotein from start codon. For V5 VLPs, tandem repeat consisting of 1’ tandem and 2’ tandem includes between 150 and 260 amino acids of G glycoprotein. PCR products for 1’, 2’ tandem, HA-TM-CT and influenza M1 were amplified, respectively. (B). Genes were cloned into pFastBacTM vectors for baculovirus production (C).