Table 1. Watermelon WRKY genes and their related information.
| Group | Subgroup | Gene name | Gene locus ID | Chromosome | Start site | End site | Genomic (bp) |
CDS (bp) |
ORF (aa) |
MW (Da) |
pI | Instability index | Aliphatic index | GRAVY | Subcellular Localization |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| I | ClWRKY13 | Cla015673 | 2 | 2511354 | 2513937 | 2584 | 1308 | 435 | 47724.69 | 5.81 | 38.39 | 64.41 | -0.861 | Nucl | |
| ClWRKY16 | Cla013402 | 2 | 29711771 | 29716746 | 4976 | 1365 | 454 | 49833.11 | 8.35 | 65.88 | 56.48 | -0.760 | Nucl | ||
| ClWRKY17 | Cla008104 | 3 | 160231 | 162530 | 2300 | 1765 | 584 | 63832.83 | 7.71 | 59.59 | 45.75 | -0.911 | Nucl | ||
| ClWRKY20 | Cla009557 | 3 | 15970670 | 15975906 | 5237 | 1944 | 647 | 70986.48 | 4.80 | 45.09 | 66.43 | -0.647 | Nucl | ||
| ClWRKY32 | Cla010216 | 5 | 31164260 | 31166896 | 2637 | 1482 | 493 | 53734.45 | 6.90 | 58.42 | 57.93 | -0.865 | Nucl | ||
| ClWRKY35 | Cla018733 | 6 | 21510656 | 21513842 | 3187 | 1542 | 513 | 55676.55 | 6.73 | 57.67 | 55.28 | -0.965 | Nucl | ||
| ClWRKY54 | Cla004492 | 10 | 4199747 | 4205383 | 5637 | 1728 | 575 | 62805.42 | 6.09 | 44.89 | 61.34 | -0.766 | Nucl | ||
| ClWRKY55 | Cla004431 | 10 | 4866664 | 4871403 | 4740 | 2295 | 764 | 82969.81 | 6.04 | 53.77 | 59.11 | -0.702 | Nucl | ||
| ClWRKY59 | Cla017851 | 10 | 26731256 | 26733477 | 2222 | 1452 | 483 | 53456.89 | 8.73 | 49.16 | 63.75 | -0.742 | Nucl | ||
| ClWRKY60 | Cla018026 | 10 | 28064812 | 28067485 | 2674 | 1428 | 475 | 51955.19 | 6.00 | 62.22 | 51.56 | -0.785 | Nucl | ||
| ClWRKY63 | Cla016540 | 11 | 22331642 | 22336203 | 4562 | 1515 | 504 | 55199.81 | 8.07 | 59.28 | 60.60 | -0.777 | Nucl | ||
| II | a | ClWRKY30 | Cla021021 | 5 | 24328741 | 24331742 | 3002 | 909 | 302 | 33770.91 | 6.34 | 41.19 | 71.36 | -0.663 | Nucl |
| a | ClWRKY49 | Cla022362 | 8 | 22951835 | 22953972 | 2138 | 924 | 307 | 33801.28 | 8.91 | 46.09 | 69.54 | -0.582 | Nucl | |
| a | ClWRKY58 | Cla017213 | 10 | 18648384 | 18649816 | 1433 | 942 | 313 | 34349.68 | 8.70 | 46.15 | 64.25 | -0.688 | Nucl | |
| II | b | ClWRKY4 | Cla008346 | 1 | 10188973 | 10192981 | 4009 | 1785 | 594 | 64179.01 | 8.15 | 44.99 | 58.23 | -0.698 | Nucl |
| b | ClWRKY7 | Cla014433 | 1 | 30737723 | 30740093 | 2371 | 1872 | 623 | 67317.35 | 6.45 | 43.91 | 58.06 | -0.752 | Nucl | |
| b | ClWRKY10 | Cla007656 | 2 | 46121 | 49115 | 2995 | 1638 | 545 | 58337.71 | 6.38 | 55.83 | 55.94 | -0.707 | Nucl | |
| b | ClWRKY28 | Cla013052 | 5 | 10364374 | 10369111 | 4738 | 1284 | 427 | 47866.14 | 8.72 | 57.37 | 55.08 | -1.007 | Nucl | |
| b | ClWRKY37 | Cla019127 | 6 | 25287085 | 25291877 | 4793 | 1611 | 536 | 58252.73 | 6.74 | 53.03 | 60.84 | -0.733 | Nucl | |
| II | c | ClWRKY1 | Cla004938 | 1 | 623905 | 625501 | 1597 | 927 | 308 | 33142.46 | 5.14 | 57.26 | 64.38 | -0.589 | Nucl |
| c | ClWRKY3 | Cla008480 | 1 | 8796142 | 8797069 | 928 | 555 | 184 | 21088.56 | 9.27 | 41.19 | 54.02 | -0.820 | Nucl | |
| c | ClWRKY8 | Cla009748 | 1 | 32574758 | 32576313 | 1556 | 780 | 259 | 28697.44 | 5.50 | 47.24 | 51.58 | -0.873 | Nucl | |
| c | ClWRKY12 | Cla007761 | 2 | 997830 | 999818 | 1989 | 741 | 246 | 28331.69 | 9.17 | 46.89 | 57.80 | -0.930 | Nucl | |
| c | ClWRKY15 | Cla013485 | 2 | 28883241 | 28885653 | 2413 | 891 | 296 | 33037.24 | 5.44 | 54.53 | 52.70 | -0.892 | Nucl | |
| c | ClWRKY21 | Cla018197 | 4 | 19676671 | 19679194 | 2524 | 993 | 330 | 35851.11 | 9.06 | 78.76 | 57.94 | -0.616 | Nucl | |
| c | ClWRKY22 | Cla021067 | 5 | 117031 | 118615 | 1585 | 672 | 223 | 25311.98 | 8.92 | 47.66 | 48.43 | -0.988 | Nucl | |
| c | ClWRKY24 | Cla021203 | 5 | 1196965 | 1198094 | 1130 | 372 | 123 | 14049.65 | 7.71 | 73.55 | 47.72 | -1.120 | Mito | |
| c | ClWRKY26 | Cla021806 | 5 | 6402958 | 6404527 | 1570 | 1047 | 348 | 38019.94 | 6.00 | 66.98 | 55.80 | -0.695 | Nucl | |
| c | ClWRKY29 | Cla002084 | 5 | 18166261 | 18168850 | 2590 | 528 | 175 | 19597.98 | 9.06 | 32.24 | 47.89 | -0.782 | Nucl | |
| c | ClWRKY34 | Cla009235 | 6 | 4823071 | 4824445 | 1375 | 936 | 311 | 34297.08 | 6.17 | 70.09 | 52.67 | -0.746 | Nucl | |
| c | ClWRKY42 | Cla014665 | 7 | 18910904 | 18913128 | 2225 | 567 | 188 | 20902.72 | 8.19 | 50.65 | 69.47 | -0.603 | Extr | |
| c | ClWRKY44 | Cla010867 | 7 | 30588853 | 30589646 | 794 | 423 | 140 | 15908.93 | 9.47 | 51.33 | 50.07 | -0.836 | Cyto | |
| c | ClWRKY47 | Cla021984 | 8 | 19308051 | 19308824 | 774 | 462 | 153 | 17791.52 | 5.07 | 51.12 | 47.65 | -1.098 | Nucl | |
| c | ClWRKY50 | Cla015154 | 9 | 3914241 | 3917158 | 2918 | 846 | 281 | 31889.45 | 6.55 | 52.03 | 58.26 | -0.746 | Nucl | |
| c | ClWRKY53 | Cla005515 | 9 | 34426506 | 34428311 | 1806 | 879 | 292 | 33087.73 | 8.06 | 59.50 | 53.77 | -0.883 | Nucl | |
| c | ClWRKY57 | Cla017345 | 10 | 17001468 | 17004493 | 3026 | 918 | 305 | 33394.38 | 6.45 | 42.48 | 60.43 | -0.567 | Nucl | |
| c | ClWRKY62 | Cla003370 | 11 | 7658790 | 7659877 | 1088 | 366 | 121 | 14204.00 | 9.46 | 41.57 | 49.83 | -1.073 | Nucl | |
| II | d | ClWRKY6 | Cla013967 | 1 | 26958654 | 26959762 | 1109 | 894 | 297 | 32337.68 | 9.64 | 48.87 | 74.24 | -0.528 | Nucl |
| d | ClWRKY14 | Cla006772 | 2 | 9026189 | 9027547 | 1359 | 870 | 289 | 31279.29 | 9.58 | 46.61 | 72.63 | -0.495 | Nucl | |
| d | ClWRKY31 | Cla020642 | 5 | 28064322 | 28065905 | 1584 | 1143 | 380 | 41065.28 | 9.60 | 50.51 | 62.63 | -0.592 | Nucl | |
| d | ClWRKY33 | Cla009969 | 5 | 33154639 | 33156285 | 1647 | 1056 | 351 | 37729.73 | 9.60 | 53.38 | 60.40 | -0.503 | Nucl | |
| d | ClWRKY36 | Cla018870 | 6 | 23022662 | 23024071 | 1410 | 837 | 278 | 30229.44 | 9.75 | 42.23 | 66.98 | -0.560 | Nucl | |
| d | ClWRKY39 | Cla006015 | 7 | 2332176 | 2333889 | 1714 | 756 | 251 | 27970.95 | 9.67 | 48.00 | 63.71 | -0.707 | Nucl | |
| d | ClWRKY51 | Cla014818 | 9 | 6327518 | 6329225 | 1708 | 1071 | 356 | 39557.25 | 9.54 | 56.93 | 79.97 | -0.478 | Nucl | |
| II | e | ClWRKY9 | Cla009853 | 1 | 33474006 | 33477082 | 3077 | 1455 | 484 | 54152.82 | 5.95 | 59.70 | 50.79 | -0.998 | Nucl |
| e | ClWRKY18 | Cla019646 | 3 | 8018421 | 8019422 | 1002 | 831 | 276 | 30132.24 | 5.15 | 45.40 | 55.83 | -0.752 | Nucl | |
| e | ClWRKY19 | Cla019756 | 3 | 9691619 | 9695338 | 3720 | 1203 | 400 | 43272.40 | 5.96 | 42.58 | 55.90 | -0.732 | Nucl | |
| e | ClWRKY25 | Cla021207 | 5 | 1219248 | 1220421 | 1174 | 996 | 331 | 36795.95 | 5.78 | 65.63 | 55.98 | -0.779 | Nucl | |
| e | ClWRKY38 | Cla002243 | 7 | 1054123 | 1056739 | 2617 | 903 | 300 | 31750.79 | 5.32 | 59.85 | 54.37 | -0.700 | Nucl | |
| e | ClWRKY56 | Cla017355 | 10 | 16854843 | 16856049 | 1207 | 954 | 317 | 35609.63 | 4.67 | 60.56 | 71.36 | -0.733 | Extr | |
| III | ClWRKY23 | Cla021170 | 5 | 925058 | 926889 | 1832 | 1017 | 338 | 37697.76 | 5.09 | 49.63 | 68.67 | -0.574 | Nucl | |
| ClWRKY27 | Cla004233 | 5 | 8987121 | 8988506 | 1386 | 1065 | 354 | 39061.06 | 5.35 | 49.07 | 62.57 | -0.620 | Nucl | ||
| ClWRKY40 | Cla007306 | 7 | 6705089 | 6711611 | 6523 | 978 | 325 | 35540.94 | 6.86 | 59.33 | 66.37 | -0.478 | Nucl | ||
| ClWRKY41 | Cla007307 | 7 | 6714426 | 6716677 | 2252 | 858 | 285 | 31505.05 | 6.36 | 44.77 | 64.14 | -0.518 | Nucl | ||
| ClWRKY45 | Cla010918 | 7 | 31145037 | 31146784 | 1748 | 1086 | 361 | 40510.40 | 5.69 | 55.65 | 53.77 | -0.897 | Nucl | ||
| ClWRKY52 | Cla015003 | 9 | 8487793 | 8489885 | 2093 | 921 | 306 | 33831.35 | 5.46 | 60.15 | 58.59 | -0.625 | Nucl | ||
| ClWRKY61 | Cla018059 | 10 | 28283762 | 28284680 | 919 | 831 | 276 | 31089.43 | 5.90 | 54.42 | 61.88 | -0.734 | Nucl | ||
| NG | ClWRKY2 | 1 | 8698894 | 8699010 | |||||||||||
| ClWRKY5 | 1 | 25736545 | 25736845 | ||||||||||||
| ClWRKY11 | 2 | 940572 | 940688 | ||||||||||||
| ClWRKY43 | 7 | 19000478 | 19003449 | ||||||||||||
| ClWRKY46 | 7 | 8331842 | 8332172 | ||||||||||||
| ClWRKY48 | 8 | 19562135 | 19567420 |
NG, no group; CDS, coding sequence; ORF, open reading frame; MW, molecular weight; pI, theoretical isoelectric point; GRAVY, grand average of hydropathicity; Nucl, nuclear; Mito, mitochondrial matrix; Extr, extracellular; Cyto, cytoplasmic.