Table 2.
Reverse Transcription–Quantitative Real-Time Polymerase Chain Reaction Primer Sequences Used in This Study
| Genes | Forward primer | Reverse primer | Product length (bp) | Annealing temperature (°C) | GenBank accession No. |
|---|---|---|---|---|---|
| Rat cardiomyogenic specific genes | |||||
| Myh6 | 5′-TTTGACATCCGCACAGAGTG-3′ | 5′-TTTCAGTTTCGGCAGTGACC-3′ | 103 | 58 | NM_017239 |
| Myh7 | 5′-AGTGTACAATGCGCAAGTGG-3′ | 5′-TGATAGGCGTTGTCAGAGATGG-3′ | 93 | 58 | NM_017240 |
| Actc1 | 5′-AGCTTTGGTGTGCGACAATG-3′ | 5′-ACGATGGATGGGAAGACAGC-3′ | 87 | 58 | NM_019183.1 |
| Tnni3 | 5′-TGAAGAGCTTCAGGACCTATGC-3′ | 5′-GGTGACTTTTGCTTCCACGTC-3′ | 88 | 58 | NM_017144.2 |
| Gata4 | 5′-AAGGCTATCCATCTCCTGTCAC-3′ | 5′-TTATGTCCCCATGACTGTCAGC-3′ | 96 | 58 | NM_144730.1 |
| Nppa | 5′-TCTTCCTCTTCCTGGCCTTTTG-3′ | 5′-AGGTGGTCTAGCAGGTTCTTG-3′ | 109 | 58 | NM_012612.2 |
| Nppb | 5′-TTAGGTCTCAAGACAGCGCC-3′ | 5′-TCCGGTCTATCTTCTGCCCA-3′ | 96 | 58 | NM_031545.1 |
| Gja1 | 5′-TATTCGTGTCTGTGCCCACC-3′ | 5′-GGCCACTTTGAGCTCCTCTT-3′ | 101 | 58 | NM_012567.2 |
| Rat reference genes | |||||
| Gapdh | 5′-AGTTCAACGGCACAGTCAAG-3′ | 5′-CCCATTTGATGTTAGCGGGATC-3′ | 99 | 58 | AF106860.2 |
| Actb | 5′-AGGCCAACCGTGAAAAGATG-3′ | 5′-AGGCATACAGGGACAACACAG-3′ | 96 | 58 | NM_031144.3 |
| G6pd | 5′-TCACACCATTGCTGCACAAG-3′ | 5′-TTCATCAGCTCATCTGCCTCTG-3′ | 97 | 58 | NM_017006.2 |
| Rplp0 | 5′-AGAACACCATGATGCGCAAG-3′ | 5′-TGAACACAAAGCCCACGTTC-3′ | 102 | 58 | NM_022402.2 |
| Human vasculogenic specific genes | |||||
| PECAM1 | 5′-TGGCAACTACACGTGCAAAG-3′ | 5′-AAGATTCCAGTTCGGGCTTG-3′ | 101 | 58 | NM_000442.4 |
| KDR | 5′-TGGCCAAGTGATTGAAGCAG-3′ | 5′-ATGCTCACTGTGTGTTGCTC-3′ | 103 | 58 | NM_002253.2 |
| TIE1 | 5′-ACGCAGCCATCAAAATGCTG-3′ | 5′-TGCCCCAATTTGCACAGAAC-3′ | 91 | 58 | NM_005424.4 |
| TEK | 5′-AGAATGCATTTGCCCTCCTG-3′ | 5′-AAGTTCTGCCAAACGTGTGC-3′ | 77 | 58 | NM_000459.3 |
| VWF | 5′-AGAAAGCCCATTTGCTGAGC-3′ | 5′-AAGTATCGCACAGCAAAGCC-3′ | 94 | 58 | NM_000552.3 |
| Human reference genes | |||||
| GAPDH | 5′-AATTCCATGGCACCGTCAAG-3′ | 5′-ATCGCCCCACTTGATTTTGG-3′ | 104 | 58 | NM_002046.4 |
| β-ACTIN | 5′-TCGTGCGTGACATTAAGGAG-3′ | 5′-TTGCCAATGGTGATGACCTG-3′ | 133 | 58 | M10277.1 |
| G6PD | 5′-TCATCATCATGGGTGCATCG-3′ | 5′-AAGGTGTTTTCGGGCAGAAG-3′ | 97 | 58 | NM_000402.4 |
| RPLP0 | 5′-AGAACACCATGATGCGCAAG-3′ | 5′-AACACAAAGCCCACATTCCC-3′ | 100 | 58 | NM_001002.3 |
Actc1, actin, alpha, cardiac muscle 1; G6pd, glucose-6-phosphate dehydrogenase; Gapdh, glyceraldehyde-3-phosphate dehydrogenase; Gja1, gap junction protein, alpha 1; KDR, kinase insert domain receptor, a type III receptor tyrosine kinase; Myh6, myosin heavy chain 6; Myh7, myosin heavy chain 7; Nppa, natriuretic peptide A; Nppb, natriuretic peptide B; Rplp0, ribosomal protein lateral stalk subunit P0; TEK, TEK tyrosine kinase, endothelial; TIE1, tyrosine kinase with immunoglobulin-like and EGF-like domains 1; Tnni3, troponin I3, cardiac type.