Fig 5.

Co-localization of IL-1β with Aβ, p-tau and GMF in temporal cortex of AD and non-AD brain. (A) Sections were immunostained with anti-IL-1β and 6E10 respectively. Representative immunofluorescence staining display the higher expression and co-localization of IL-1β (green) and 6E10 (red) in AD as compared with non-AD. (C) Sections were immunostained withanti-IL-1β and with anti-p-tau respectively to check the co-localization of IL-1β with p-tau. Representative immunofluorescence staining display the higher expression and co-localization of IL-1β (green) and p-tau (red) in AD compared with Non-AD (E) Sections were immunostained with anti-IL-1β and anti-GMF respectively to check the co-localization of IL-1β with GMF. Representative immunofluorescence staining display the higher expression and co-localization of IL-1β (green) and GMF (red) in AD compared with non-AD. Data are expressed as mean ± standard error, from each group (n= 3–5). *p <0.05 versus non-AD was considered statistically significant. Zoom of boxed area showed the enlarge view of co-localization of IL-1β (green) with 6E10 (red), p-tau (red) and GMF (red) Scale bar = 50 μm and AU (Arbitrary Unit). (B, D, F) Quantification of IL-1β, 6E10, p-tau and GMF based on average labeled intensity and labeled positive area in AD and non-AD brains.