The autophagy marker SQSTM1/p62 accumulates and co-localizes with Aβ marker 6E10 p-tau and GMF in the temporal cortex of human AD compared to non-AD brain. (A) Sections were double immunofluorescence stained with anti-SQSTM1/p62 (red) and 6E10 (green), respectively and displayed higher accumulation and co-localization of SQSTM1/p62 and 6E10 in AD compared with non-AD. (C) Sections were immunostained with anti-SQSTM1/p62 and anti-p-tau respectively and displayed higher accumulation and co-localization of SQSTM1/p62with p-tau in AD compared with non-AD (E) Sections were immunostained with anti-SQSTM1/p62 and anti-GMF respectively and displayed higher accumulation and co-localization of SQSTM1/p62 with GMF in AD compared with non-AD. Data were analyzed as mean ± standard error, from each group (n= 3–5). *p <0.05 versus non-AD was considered statistically significant. Zoom of boxed area showed the enlarge view of co-localization of SQSTM1/p62 (red) with 6E10 (green), p-tau (green) and GMF (green) Scale bar = 50 μm and AU (Arbitrary Unit). (B, D, F) Quantification of SQSTM1/p62, 6E10, p-tau and GMF based on average labeled intensity and labeled positive area in AD and non-AD brains.