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. Author manuscript; available in PMC: 2018 Jan 16.
Published in final edited form as: Biochemistry. 2016 May 18;55(21):3048–3059. doi: 10.1021/acs.biochem.6b00429

Figure 6.

Figure 6

Titration of wild type W1 followed at indicated backbone amide site by heteronuclear NMR (A, D; combined chemical shift34 perturbation based on our previously reported data12) and of indicated 5F-Trp-labeled mutants followed by 19F NMR, with absolute (B, E) and normalized (C, F) 19F chemical shift changes illustrated (spectra shown in Figure S8). W1-Avg. represents average from all assigned residues (± average deviation). Dashed lines are used to join titration points when signals could not be tracked due to severe line broadening.