. Author manuscript; available in PMC: 2018 Jan 16.

Published in final edited form as: Biochemistry. 2016 May 18;55(21):3048–3059. doi: 10.1021/acs.biochem.6b00429

Table 2.

⁹F chemical shifts (±0.01 ppm; δH₂O in 90%H₂O+10%D₂O and δD₂O in 10%H₂O+90%D₂O), SIS (Δ(δD₂O - δH₂O)), T₁ relaxation time constant, and linewidth for 5F-Trp-labeled W₁ mutants alongside TFE and 5-fluoroindole controls.

	R36W	F90W	F146W	Y169W	TFE	5-fluoroindole
δH₂O (ppm)	−123.64	−126.27	−124.48	−124.73	−76.53	−125.48
δD₂O (ppm)	−123.81	−126.23	−124.54	−124.82	−76.64	−125.67
SIS (ppm)	−0.17	+0.04	−0.06	−0.09	−0.11	−0.19
T1 (s)	0.64±0.18	0.44±0.06	0.45±0.05	0.61±0.07
Linewidth (Hz)	38.2±0.9	44.8±0.9	25.3±1.1	19.5±0.7