Table 1.
HSC Activity Is Enhanced Extrinsically
| GP+E86 (virus)
|
NIH 3T3 (no virus)a
|
|||
|---|---|---|---|---|
| %GFP | %CD45.1 | %GFP | %CD45.1 | |
| vector | 62 ± 26 | 4 ± 1 | 0 ± 0 | 5 ± 2 |
|
| ||||
| Fos | 71 ± 9 | 31 ± 8 | 0 ± 0 | 35 ± 14 |
|
| ||||
| Sfpi1 | 37 ± 17 | 26 ± 12 | 0 ± 0 | 2 ± 1 |
|
| ||||
| Tcfec | 48 ± 21 | 22 ± 8 | 0 ± 0 | 13 ± 5 |
|
| ||||
| Hmgb1 | 22 ± 14 | 20 ± 10 | 0 ± 0 | 14 ± 5 |
Donor-derived reconstitution levels (%CD45.1+) observed 16 weeks after transplantation in mice transplanted with cells cocultured either with virus-producing cells (GP+E-86) or similar cells not producing virus (NIH 3T3), both overexpressing selected genes. Values are presented as mean ±SEM of independent experiments (n) per candidate gene, where n = 4, except for control vector, where n = 8. For each independent experiment, a mean of three mice per gene was evaluated.
a
Mice were analyzed at 16 (experiments 1 and 2) and 8 (experiments 3 and 4) weeks after transplantation; %GFP, gene transfer assessed by flow cytometry.