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. Author manuscript; available in PMC: 2018 Feb 6.
Published in final edited form as: Oncogene. 2017 Oct 9;37(5):651–662. doi: 10.1038/onc.2017.372

Figure 4.

Figure 4

Hypoxia represses CDH1 mRNA translation and activates CDH22 synthesis in an eIF4E2-dependent manner. (a–c) The polysomal association of CDH1 and CDH22 mRNAs was observed by reverse transcriptase (RT)–PCR in parental (a), control cells stably expressing a non-targeting shRNA (b), or shRNA targeting eIF4E2 mRNA (KD1.2) (b), KD cells stably expressing an exogenous empty vector (KD Ctrl) (c), or the exogenous eIF4E2 coding sequence (Exo1 4E2) (c) under the indicated oxygen conditions. (d) The polysomal association of CDH1 and CDH22 mRNAs was observed by RT–PCR in parental cells exposed to normoxia or hypoxia and the mTORC1 inhibitor Torin 1 for 2 h. Images are representative of at least three biological replicates. All experiments performed in MDA-MB-231 breast carcinoma.