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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Mol Cancer Res. 2017 Aug 15;15(11):1469–1480. doi: 10.1158/1541-7786.MCR-17-0280

Figure 6. Expression of Vav3 DH domain inhibits anchorage dependent and anchorage independent growth, and induces apoptosis in AR-expressing cells.

Figure 6

A) The androgen-dependent cell line LNCaP, the CRPC cell lines 22Rv1 and CWR-R1, and the AR-null PC3 cell line stably expressing DH-FLAG or empty vector linked to FLAG (EV-FLAG) were transfected with a non-perturbing nuclear restricted green fluorescent label and incubated in an Incucyte Zoom (Essen Bioscience), acquiring phase and green fluorescent images. Graphs show the mean ± SE for the 12 replicates for each condition. B) CWR-R1 and PC3 cells stably expressing DH-FLAG or empty vector linked to FLAG (EV-FLAG) were transfected with an apoptosis marker reagent, which is cleaved by activated caspase 3/7, releasing a green fluorescent label. Cells were then incubated in an Incucyte Zoom, acquiring phase and green fluorescent images. Graphs show the mean ± SE for the 6 replicates for each condition. C) CWR-R1 and the AR-null PC cell line ALVA31 stably expressing DH-FLAG or empty vector were assessed for soft agar growth. Average colony number per plate ± SEM was plotted. Data represent two independent experiments performed in triplicate. (Student’s T-test, p value<0.01).