Fig. 6.

Increase in HIV-1 NAb responses in mice primed with TM1 modified Envs. NAb titers against HIV-1 MN.3 and SF162.LS pseudoviruses at the indicated time points were measured using TZM-bl reporter cells. The mean ID₅₀ titers are shown for (A) 89.6 N7 and (B) JRFL Env immunization series. N = 8–10 mice per immunized group. Background activity against a murine leukemia virus Env pseudovirus is subtracted from all data except the naive time point in (A), for which serum volume was limited. Error bars indicate the SEM, and asterisks denote a significant difference (p < 0.05) in NAb titer between WT and TM1 CT groups by Kruskal-Wallis test with Dunn's correction.