Fig S2.

No significant difference in VACV-specific CD8⁺or Env-specific CD4⁺T cell responses resulting from TM1 CT modification. Splenocytes from mice immunized with 89.6 N7 Envs with WT or TM1 CT were stimulated with peptide pools from (A) VACV or (B) SHIV 89.6P Env and were stained for intracellular cytokine expression. Data represent mean frequencies of IFNγ⁺ and TNFα⁺ cells, as a percentage of total CD8⁺ or CD4⁺ T cells, and error bars are the SEM; n.s. = not significant (p > 0.05) by Mann-Whitney test.