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. 2018 Jan 2;22(1):44–58. doi: 10.1016/j.celrep.2017.12.037

Figure 1.

Figure 1

Interaction Profiling of ZC3H18 Identifies Histones

(A) SDS-PAGE gel of proteins co-precipitating with ZC3H18-3xF at the indicated buffer compositions. Two major isoforms of the ZC3H18-3xF bait protein are indicated with red and green dots for the fast- and slow-migrating forms, respectively. Denoted with color code are also CBCN complex components MTR4, ARS2, ZCCHC8, and CBP80 as well as histones. The identity of the indicated bands was established by MS analysis.

(B and C) Scatterplots presenting MS analysis of co-purified CBCN components (B) and core histones (C) in the ZC3H18-3xF AC experiments performed in 100, 300, and 500 mM NaCl-containing buffers (indicated by red, blue, and green dots, respectively). Exact buffer compositions are shown below the plots. The y axes display protein abundances estimated as the ratio between a protein’s mean peptide intensity from two biological experiments and its molecular weight and normalized to the abundance of ZC3H18-3xF bait protein. The x axes display RNase A/T1 resistance calculated as the ratio between protein abundances in RNase A/T1-treated versus untreated samples.