Figure 4.

miR-483-5p post-transcriptionally downregulates ALCAM expression by directly targeting its 3′-UTR. (A) ALCAM mRNA levels were repressed by miR-483-5p in HCC cells. (B) ALCAM protein levels were repressed by miR-483-5p mimics in HCC cells. (C) ALCAM protein levels were up-regulated in HCC cells with miR-483-5p inhibitor. (D) Diagram of putative miR-483-5p binding sites in the 3′-UTR of ALCAM. The mutant sequences ALCAM 3′-UTR used in the luciferase reporter constructs are indicated in red. (E) Relative activities of luciferase reporters containing ALCAM 3′-UTR variants co-transfected with miR-483-5p or negative control mimics in HEK 293T cells. ^*p < 0.05; ^***p < 0.001.