FIGURE 2.

Effect of bergenin on expressions of pro-inflammatory cytokines and barrier function-associated proteins in colons of mice with DSS-induced colitis. Mice were treated with 2.5% DSS in drinking water for 7 days, provided with drinking water for another 3 days. Bergenin (20, 50 mg/kg; i.g.) and 5-ASA (100 mg/kg; i.g.) were administrated daily for consecutive 10 days. (A) The mRNA expressions of pro-inflammatory cytokine IL-1β, IL-6, TNF-α and barrier function-associated proteins occludin, E-cadherin, and MUC-2 in colons were determined by Q-PCR assay. (B) Protein levels of IL-6 and TNF-α were determined by ELISA. Data were presented as the means ± SEM (n = 6). ^##p < 0.01 vs. the group without any treatment; ^∗p < 0.05 and ^∗∗p < 0.01 vs. DSS group.