FIGURE 4.

Effect of bergenin on the activation of NF-κB signaling pathway in macrophages. RAW264.7 cells were treated with bergenin (3, 10. 30 μM) for 24 h, and stimulated with LPS (1 μg/mL) for another 30 min. (A) Levels of phosphorylated IKKβ and IκBα were determined by western blotting assay. The β-actin was used as control. (B) Level of phosphorylated NF-κB-p65 and nuclear translocation of NF-κB-p65 were determined by western blotting assay. The β-actin and Lamin B1 were used as controls. (C) DNA-binding activity of NF-κB-p65 was determined by EMSA. Data were presented as means ± SEM of three independent experiments. ^##p < 0.01 vs. the group without any treatment; ^∗p < 0.05 and ^∗∗p < 0.01 vs. LPS group.