Skip to main content
. 2018 Jan 12;8:981. doi: 10.3389/fphar.2017.00981

FIGURE 6.

FIGURE 6

Effect of PPARγ antagonist and siPPARγ on bergenin-mediated inhibition of NF-κB-p65 nuclear translocation in macrophages. (A) RAW264.7 cells were incubated with GW9662 (10 μM) for 1 h, followed by treatment of bergenin (30 μM) for another 24 h, and stimulated with LPS for 30 min. Protein level of NF-κB-p65 were determined by western blotting assay. (B) RAW264.7 cells were transfected with siPPARγ for 24 h, followed by treatment of bergenin (30 μM) for another 24 h, and stimulated with LPS for 30 min. Protein levels of NF-κB-p65 in cytosol and nuclear were determined by western blotting assay. (C) RAW264.7 cells were incubated with GW9662 (10 μM) for 1 h, followed by treatment of bergenin (30 μM) for another 24 h, and stimulated with LPS for 30 min. Cells were immunostained with DAPI (blue) and NF-κB-p65 (red), and then observed by using a fluorescence microscope. Data were presented as means ± SEM of three independent experiments. ##p < 0.01 vs. the group without any treatment; ∗p < 0.05 and ∗∗p < 0.01 vs. LPS group; $p < 0.05 and $$p < 0.01 vs. Bergenin group; &p < 0.05 and &&p < 0.01 vs. RGZ group. RGZ, rosiglitazone.