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. 2018 Jan 12;8:980. doi: 10.3389/fphar.2017.00980

FIGURE 4.

FIGURE 4

ZEB2 is a direct target of miR-145 in activated HSCs cells. (A) The target genes of miR-145 were predicted by using bioinformatics tools (TargetScan, CLIP-Seq, miRDB, and miRanda). (B) A miR-145 mimic or negative control and a luciferase vector encoding the wild-type or mutant ZEB2 3′-UTR region were co-transfected into LX-2 cells, and the relative luciferase activity was measured. (C) Quantitative real-time PCR analysis for mRNA expression of ZEB2 after transfected with miR-145 mimics, miR-145 inhibitor, or miR-145 NC. (D) Western blot analysis for protein expression of ZEB2 after transfected with miR-145 mimics, miR-145 inhibitor, or miR-145 NC. (E) Protein level of the cleaved of caspase-3 was determined by Western blot analysis in activated LX-2 cells co-transfected with miR-145 inhibitor and ZEB2-siRNA, The assays were performed at least three times with similar results. Data are shown as the mean ± SD (n = 3) of one representative experiment. ∗p < 0.05 versus normal groups.